By Frédéric De Ceuninck (Editor), Massimo Sabatini (Editor), Philippe Pastoureau (Editor)
An vital choice of up to date classical and rising options that promise so as to add severe wisdom to our knowing of cartilage metabolism in well-being and disorder. quantity 1: mobile and Molecular instruments describes confirmed molecular and mobile strategies for the in vitro research of standard and osteoarthritic cartilage via biochemical, biomolecular, immunological, and actual methods, with emphasis at the genetic manipulation of cells. quantity 2: constitution and In Vivo research, deals state of the art approaches for the study-at the tissue level-of turnover, constitution, and functioning of standard and diseased articular cartilage through invasive and noninvasive potential. finished and up to date, the 2 volumes of Cartilage and Osteoarthritis offer researchers and bench scientists with easily reproducible protocols for brand new experiments to understand-from the mobile to the animal level-the pathophysiology of cartilage and to find molecular pursuits for pharmacological intervention.
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Additional resources for Cartilage and Osteoarthritis Vol 2 Structure and In Vivo Analysis (Methods in Molecular Medicine)
64, 403–434. 6. 6 Myllyharju, J. and Kivirikko, K. I. (2001) Collagens and collagen-related diseases. Ann. Med. 33, 7–21. 7. 7 Mundlos, S. and Olsen, B. R. (1997) Heritable diseases of the skeleton. Part II: Molecular insights into skeletal development-matrix components and their homeostasis. FASEB J. 11, 227–233. 8. Jacenko, O. and Olsen, B. R. (1995) Transgenic mouse models in studies of skeletal disorders. J. Rheumatol. 43 (suppl), 39–41. 9. 9 Olsen, B. R. (1995) Mutations in collagen genes resulting in metaphyseal and epiphyseal dysplasias.
No. 314492F), fast green FCF (CI 42053), picric acid (BDH); Ponceau de Xylidene (CI 16150), hematoxylin (CI 75290), acid fuchsin (CI 42685), phosphotungstic acid (Sigma) (see Note 5). Bovine testicular hyaluronidase (Sigma). Mounting media (Aquaperm, Eukitt). Monoclonal antibodies to type II collagen (clone II-4CII) (7) (ICN Biochemicals, N. Ryde, NSW, Australia). DAKO LSAB-2 biotinylated secondary antibody cocktail, goat–antirabbit, goat– antimouse immunoglobulins (cat. no. K0690); DAKO LSAB+ streptavidin-conjugated horseradish peroxidase (cat.
1. Morphologies of the femoral condyle and tibial plateau cartilages of laterally meniscectomized ovine stifle joints 6 mo post meniscectomy (A, B) and a control sham operated joint for comparison (C, D). The arrows and asterisks in (A) and (B) depict areas of focal damage to the weight-bearing surfaces of the laterally meniscectomized joints and osteophyte formations in the joint margins, which frequently accompany these changes in the meniscectomized joints. The sham operated control stifle joint surfaces presented in (C) and (D) have smooth, shiny, well-lubricated articulating surfaces and no marginal osteophytes.