By Ziad El Rassi
Carbohydrates and glycoconjugates play an immense position in numerous lifestyles procedures. the wide range of carbohydrate species and their inherent polydispersity and heterogeneity require separation recommendations of excessive resolving strength and excessive selectivity resembling excessive functionality liquid chromatography (HPLC) and capillary electrophoresis (HPCE). within the final decade HPLC, and lately HPCE tools were constructed for the excessive answer and reproducible quantitation of carbohydrates. regardless of the significance of those column separation applied sciences within the quarter of carbohydrates, no earlier publication describes really good equipment for the separation, purification and detection of carbohydrates and glycoconjugates by means of HPLC and HPCE.
Therefore, the target of the current ebook is to supply a entire assessment of carbohydrate research by way of HPLC and HPCE via masking analytical and preparative separation concepts for all periods of carbohydrates together with mono- and disaccharides; linear and cyclic oligosaccharides; branched heterooligosaccharides (e.g., glycans, plant-derived oligosaccharides); glycoconjugates (e.g., glycolipids, glycoproteins); carbohydrates in meals and beverage; compositional carbohydrates of polysaccharides; carbohydrates in biomass degradation; etc.
The booklet might be of curiosity to a large viewers, together with analytical chemists and biochemists, carbohydrate, glycoprotein and glycolipid chemists, molecular biologists, biotechnologists, and so forth. it is going to even be an invaluable reference paintings for either the skilled analyst and the newcomer in addition to for clients of HPLC and HPCE, graduates and postdoctoral scholars.
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Extra info for Carbohydrate analysis: high performance liquid chromatography and capillary electrophoresis
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A small fraction of the galactolipid in chloroplasts contains a 6-sulfonyl group on the galactose. In the purification of glycolipids , total lipids are usually extracted from whole wet tissue by homogenization in at least 20 volumes of chloroform-methanol, 2 : 1, References pp. 35-37 Chapter I 12 in a Waring blender for three minutes. Some modification of the extraction medium is necessary for complete extraction of the more complex glycolipids. Addition of water to the clarified organic phase allows partitioning of the highly polar glycolipids into the aqueous phase.