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By Amitava Mukherjee

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Thus, successful immobilization relies almost exclusively on coated electrodes. Surface coating needs to be well defined in terms of chemical functionalities and physical properties. Self assembled monolayers (SAMs) of alkanethiols are among the most popular biocompatible coatings employed in studies of interfacial interactions for addressing fundamental aspects of heterogeneous ET, but also molecular recognition and cell growth processes, heterogeneous nucleation and crystallization, biomaterial interfaces, etc (Ulman, 2000).

36 Biomimetics, Learning from Nature aa3 cytochrome c oxidase: The CcO from Rhodobacter sphaeroides is a member of the type A family of heme copper oxygen reductases that houses three redox centers in the catalytic subunit (subunit I) and a dinuclear copper CuA in the subunit II (Figure 4B). , 2008). The protein was specifically attached, uniformly oriented and catalytically active in the biomimetic construct. The orientation of the attached protein could be controlled since the His-tag was introduced into the amino acid sequence of R.

On the one hand, the B2 state has a reduction potential which is more than 300 mV lower than that of the native protein (denoted as state B1) as determined by potential-dependent SERR spectroscopy (Murgida and Hildebrandt, 2004a). , 2009). , 2009). Degradation of cardiolipin increases the permeability of the membrane, thus facilitating the transfer of Cyt-c to the cytosol where it binds to Apaf-1, in one of the initial events of the cell apoptosis. Thus, it is likely that the switch from the “normal” redox function of Cyt-c (B1 state) to the apoptotic function (B2 state) depends on the local electric field which is, in turn, modulated by the membrane potential.

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